Microchromatographic column and method

Details Microchromatographic column and method Microchromatographic column and method

1980-12-16

1982-07-27

Adee, John

Liquid purification or separation

Processes

Chromatography

2101982, B01D 1508

Patent

active

043416357

ABSTRACT:
A liquid microchromatographic column having a tubular reservoir portion receiving the fluid solution to be separated and discharging into a barrel portion containing finely divided ion exchange particles for separating the solution. A cap having an inlet port is received in sealed relation on the reservoir portion open end. The inlet port of the cap has a smooth generally cylindrical internal surface for receiving the discharge tip of a microsyringe or other pressure source, in sealed relation. The chromatographic method of this invention then includes introducing the fluid solution to be separated and an eluting solution into the reservoir. The discharge tip of a microsyringe is then inserted into the inlet port of the cap and the plunger of the syringe is depressed to increase the pressure in the reservoir and create a pressure drop across the ion exchange particles, substantially reducing the time required for chromatographic separation.

REFERENCES:
patent: 3250395 (1966-05-01), Blume
patent: 3922223 (1979-11-01), Burkhartsmeier
patent: 4112743 (1978-09-01), Mowery, Jr.

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