Microchip optical transport technology for use in a personal flo

Optics: measuring and testing – By particle light scattering – With photocell detection

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356337, 356 73, 356317, 422108, 422 70, 422 681, 422 81, G01N 2100, G01N 3002

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active

060974850

ABSTRACT:
An ultra-miniature personal flow cytometer (pFCM) for the measurement of laser-induced fluorescence. The pFCM is approximately the size of a fluorescent microscope and includes a miniature optical module onto which the flow cytometry components are coupled. The miniature optical module is closely integrated with a flow cell through which a sample cell stream flows. The advantage of the pFCM is its compact size and efficiency that is made possible due to the unique microchip demultiplexing waveguide utilized in the separation of fluorescent wavelengths. The microchip demultiplexing waveguide is wholly contained in a tiny microchip consisting of a wave channel layer, a buffer layer, a substrate layer, and a plurality of micrograting filters. Photodetectors may either be coupled to the microchip demultiplexing waveguide by individual fiber optic members or incorporated into the wave channel layer in a photodiode array. The pFCM has wide ranging applications, but in particular is very useful in the analysis of sperm chromatin structure assay (SCSA) because of its small size and simplicity in operating.

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