Microcapillary-based flow-through immunosensor and displacement

Details Microcapillary-based flow-through immunosensor and displacement Microcapillary-based flow-through immunosensor and displacement

1997-04-28

2000-02-01

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

422 55, 422 59, 422 8208, 435 71, 435 793, 435 794, 435 795, 4352877, 4352878, 4352879, 4352887, 435808, 435 6, 436518, 436527, 436535, 436541, 436164, 436172, 436805, G01N 33558

Patent

active

060202092

ABSTRACT:
A displacement-type flow immunoassay is performed using a microcapillary sage. The inner wall of the microcapillary passage has immobilized thereon antibodies to the antigen of interest. Labeled antigen is immunologically bound to the immobilized antibodies. Sample antigen passing through the column displaces the labeled antigen. Downstream, the displaced labeled antigen is detected. The microcapillary format of the present invention enhances the sensitivity of the immunoassay over the sensitivity of displacement-type flow immunoassays performed in a column at similar flow rates.

REFERENCES:
patent: 5183740 (1993-02-01), Ligler et al.
patent: 5741639 (1998-04-01), Ensing et al.

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