Microbiologically produced N-acetyl-2,3-didehydroleucine acylase

Details Microbiologically produced N-acetyl-2,3-didehydroleucine acylase Microbiologically produced N-acetyl-2,3-didehydroleucine acylase

1990-02-01

1992-07-28

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435822, 4352521, 435116, C12N 980, C12N 100, C12N 112, C12P 1306

Patent

active

051340739

ABSTRACT:
A novel N-Acetyl-2,3-didehydroaminoacid-acylase is obtained by cultivating Zoogloea ramigera DSM 4306. The new enzyme can be used in a coupled enzyme system with an L-Leucinedehydrogenase for the enzymatic conversion of N-Acetyl-2,3-didehydroleucine to L-Leucine, D- or L-tryptophylglycine to D- or L- tryptophaneamide and glycine, as well as other tryptophanedipeptides to tryptophaneamides and free amino acids.

REFERENCES:
Nishida et al., Enzyme Microb. Technol., vol. 9, pp. 479-483, 1987.
Hummel et al., Appl. Microbiol. Biotechnol., vol. 27, pp. 283-291, 1987.

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