Microbiological scaled sterility test strips and method

Textiles: fiber preparation

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195127, C12K 100

Patent

active

040873269

ABSTRACT:
A method and apparatus for obtaining a permanent visual record of the microbiological effectiveness of a sterilizing cycle is provided by a spore carrier strip having a plurality of individual spore colony sites defined on the surface thereof, each of said sites containing a different population of spores, as for example, 10.sup.2, 10.sup.3, 10.sup.4, 10.sup.5, and 10.sup.6. The spore carrier is exposed to the sterilizing cycle, then incubated in a sterile nutrient culture medium containing a color indicator for viable microorganisms such as 2,3,5-triphenyl tetrazolium chloride. The development or lack of development of color on each spore colony site of successively increasing spore populations indicates the degree of effectiveness of the sterilizing cycle. The developed color is permanent and the carrier strip may be retained to provide a permanent visual record of the effectiveness of the sterilizing cycle.

REFERENCES:
patent: 3107204 (1963-10-01), Brown et al.
patent: 3341427 (1967-09-01), Evans et al.
patent: 3346464 (1967-10-01), Ernst
patent: 3711378 (1973-01-01), Kereluk
patent: 3819490 (1974-06-01), Klingstrom et al.

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