Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1985-02-19
1989-07-25
Warren, Charles F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435939, 260413, C12P 764
Patent
active
048513431
ABSTRACT:
Production of .gamma.-linolenic acid, by growth of a diffused mycelial inoculum of Rhizopus arrhizus in a stirred, aerated nutrient medium containing a carbohydrate energy source and an inorganic nitrogen source at a carbon:nitrogen ratio of 20:1 to 60:1 maintained at a temperature of 25.degree. C..+-.2.degree. C. and a pH of 3.75 to 6.25 in the presence of a foam breaker, until a .gamma.-linolenic concentration of at least 1.0 g/liter of medium is reached, harvesting the organism, and extraction of lipid containing the .gamma.-linolenic acid.
REFERENCES:
The Merck Index, 10th Edition, 1983, p. 790.
Gellerman et al, Biochim. et Biphys. Acta, vol. 573, (1975), pp. 23-30.
Ratledge, Progress in Industrial Microbiology, vol. 16, (1980), pp. 119-206.
Herbert Rodney A.
Keith Stephen M.
Efamol Limited
Greason Carolyn S.
Warren Charles F.
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