Microbiological process for the preparation of a protein by cult

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4352523, 43525233, 4351721, 435 698, 935 41, 935 56, 935 60, 935 73, 935 61, C12P 2100, C12N 1500, C12R 107, C12R 1185

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049450476

ABSTRACT:
The invention relates to a microbiological process for the preparation of a protein by culturing a mutant bacterial strain.
This process consists in using a bacterial strain carrying a mutation which limits, or even suppresses, the expression of the operons whose transcription depends on the fixation of the cAMP-CRP complex, a DNA sequence coding for a protein precursor having been introduced into the said bacterial strain.

REFERENCES:
Dunlap et al., 1985, J. Bacteriol., 164(1):45-50.
Amann et al., 1983, Gene, 25:167-178.
J. Daniel; Fems Microbiology Letters, vol. 24, 1984, pp. 215-218.
G. L. Gray et al.; Gene, vol. 39, No. 2/3, 1985, pp. 247-254.
R. Utsumi; Agricultural and Biological Chemistry, vol. 48, No. 8, 1984, pp. 2129-2130.
A. H. Koop et al.; Gene, vol. 28, 1984, pp. 133-146.

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