Microbiological oxidation reactions using purified monooxygenase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

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435148, 435156, 435157, 435822, 435189, C12P 1702, C12P 726, C12P 722, C12P 704, C12N 902

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045872163

ABSTRACT:
A purified hydroxylase enzyme component A of the methane monooxygenase enzyme isolated from the soluble fraction of Methylobacterium organophilum (CRL.26) (NRRL B-11,222) is found to contain three subunits. Any component A derived from methylotrophs having the particular characteristics of this isolated component A may be employed in conjunction with the flavoprotein component C of the methane monooxygenase enzyme, preferably the flavoportein component derived from the same organism, to catalyze the oxidation of various oxidizable organic substrates to their respective oxidation products. Preferably, the substrate is propylene.

REFERENCES:
patent: 4269940 (1981-05-01), Patel et al.
Dalton in Advances in Applied Microbiology, vol. 26, pp. 71-87 (1980).
Colby et al, in Biochemical Journal, vol. 171, pp. 461-468.
Stirling et al, Biochemical Journal, vol. 177, pp. 361-364.
O'Connor et al, in Journal of General Microbiology, vol. 101, pp. 327-339 (1977).

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