Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Geomicrobiological testing
Patent
1990-06-22
1992-03-03
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Geomicrobiological testing
435 25, 435810, C12Q 164
Patent
active
050932365
ABSTRACT:
This invention provides methods and systems for prospecting for subterranean oil and gas deposits by detecting the presence of hydrocarbon-consuming microorganisms in soil samples taken from a field of interest for production. A preferred method of this invention comprises suspending and diluting each soil sample in mineral salt solution, combining the sample with a selective growth, substrate such as an alcohol or aldehyde, and an oxidation-reduction dye in a gas-tight tube, incubating the sample and measuring the color change of the dye. Advantages of this system include: results obtained in short times (24 to 72 hours), substantially eliminates false positives, accurate results particularly when a spectrophotometer is used to measure the color changes, and it is automatable with computer controlled systems for processing samples, reading the color changes and calculating and plotting test results.
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Hitzman, Developments in Industrial Microbiology, vol. 2, "Comparison of Geomicrobiological Prospecting Methods Used by Various Investigators", Plenum Press, N.Y.
Gonzales-Prevatt Victoria
Munnecke Douglas M.
Genecor International, Inc.
Geo-Microbial Technologies, Inc.
Rosen Sam
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