Microbial susceptibility assay using 7-N-(aminoacyl)-7-amido-4-m

Details Microbial susceptibility assay using 7-N-(aminoacyl)-7-amido-4-m Microbial susceptibility assay using 7-N-(aminoacyl)-7-amido-4-m

1990-06-20

1991-11-12

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 18, 435 19, 435 29, 435 34, 436164, 436172, 436800, 436805, C12Q 118, C12Q 102

Patent

active

050647563

ABSTRACT:
A method of determining the susceptibility of microorganisms to an antimicrobial substance wherein the microorganisms are cultured in the presence of the antimicrobial substance, and growth or lack of growth is assessed by detecting a fluorescent product resulting from the enzymatic hydrolysis of a fluorogenic substrate. The fluorogenic substrates include 7-N-(aminoacyl)-7-amido-4-methylcoumarin, 7-N-(alanyl)-7-amido-4-methylcoumarin, or a combination of 7-N-(alanyl)-7-amido-4-methylcoumarin and 4-methyl umbelliferyl phosphate.

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