Microbial retrieval and sampling method

Details Microbial retrieval and sampling method Microbial retrieval and sampling method

1993-08-30

1994-11-01

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435294, 422100, C12Q 124

Patent

active

053607210

ABSTRACT:
This invention (FIGS. 1-4) relates to a pipette-syringe combination employing a wire probe attached to the syringe plunger to remove a cover provided over the pipette orifice. One edge of the protective cover is attached via an elastomeric strap to the pipette. When the cover is forced away from the pipette orifice, the elastomeric strap pulls the cover away from, and prevents return of the cover over, the pipette orifice. A suitable sample may then be drawn in the pipette at the location of the cover removal. In the embodiment of FIGS. 5-7, the protective cover is manually removed to obtain a surface growth sample by the wire probe and the sample retracted within the pipette. A quantity of nutrient broth is added to the pipette, the pipette sealed by manually replacing the protective cover, with subsequent incubation of the sealed pipette in an incubator. After incubation, the pipette is agitated to distribute the growth, the cover is manually removed and controlled quantities of the broth transferred to a number of tubes for further study.

REFERENCES:
patent: 3603156 (1971-09-01), Konkol
patent: 3732734 (1973-05-01), Avakian
patent: 3838013 (1974-09-01), Bergeron
patent: 3991617 (1976-11-01), d'Autry
patent: 4009611 (1977-03-01), Koffer
patent: 4054491 (1977-10-01), Lindgren
patent: 4151750 (1979-05-01), Suovaniemi
patent: 4248830 (1981-02-01), Kallies et al.
patent: 4283950 (1981-08-01), Tervamaki
patent: 4487081 (1984-12-01), De Vaughn et al.

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