Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1997-03-31
2000-07-11
Marx, Irene
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
435108, 4352521, 4352533, C12P 1304
Patent
active
060871369
ABSTRACT:
A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial microorganisms (NCIM), National Chemical laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30.degree. C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.
REFERENCES:
patent: 4094741 (1978-06-01), Yamada
patent: 4111749 (1978-09-01), Degen
Sun, Wanru., Acta Microbiol Sin, (1983) 23 (2), 133-142.
Bastawde Kulbhushan Balwant
Gaikwad Bhaskar Ganapatrao
Gokhale Digambar Vitthal
Jogdand Vithal Venkatrao
Joshi Ramesh Anna
Council of Scientific & Industrial Research
Marx Irene
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