Microbial process for detection of toxic substances

Details Microbial process for detection of toxic substances Microbial process for detection of toxic substances

1995-01-20

1997-09-30

Paden, Carolyn

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 29, C12Q 102

Patent

active

056724705

ABSTRACT:
A microbial process for detection of toxic substances. The bacterial luminescence test so far used to detect toxic substances is extremely fast in its performance and reacts with very high sensitivity to heavy metals such as mercury and lead. However, that is not the case for other toxicologically relevant heavy metals such as cadmium and chromium. The inhibitory effect of toxic substances on the phosphotransferase system of bacteria, in particular, the E. coli mutant 1219 bgl+, is set forth as proof for the presence of toxic substances in samples. This test method responds with high sensitivity to the presence of the heavy metals cadmium and chromium. The test process constitutes a complement to the bacterial luminescence test and makes it possible to quickly exclude the presence of toxicologically relevant concentrations of cadmium and chromium in such cases where only a slight or no effect at all is indicated on the phosphotransferase system.

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G. Tesoriere et al. "The Purification and Properties of Nucleoside Phosphotransferase From Mucosa of Chicken Intestine", 231-244, 1984, Biochimica et Biophysica Acta.
V.L. Crow et al. "The Effect of Monovalent and Divalent Cations on the Activity of Streptococcus Lactis C10 Pyruvate Kinase", 105-114,1977, Biochimica et Biophysica Acta. L. Ciskanik et al. Inhibition and Inactivation of Pyruvate Phosphate Dikinase with Cr(III).
"Complexes of Adenosine 5'-Triphosphate and Inorganic Pyrophosphate", 113-125, 1986, J. Enzyme Inhibition, vol. 1.
I.A. Brand et al. "Zn2+-dependent Reversible Inactivation of Rat Liver Phosphofructokinase-1", 5892-5900, 1986, The Journal of Biological Chemistry.

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