Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-03-03
1996-10-22
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 732, 435 32, 435 39, 435968, 436172, 436800, C12Q 118
Patent
active
055675980
ABSTRACT:
This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.
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Burrell Gregory J.
Hu Kwok-Yu
Monthony James F.
Sapitowicz Robert
Stitt David T.
Becton Dickinson and Company
Cano Milton I.
Capello Susan A.
Kepplinger Esther M.
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