Microbial method of producing inositol

Details Microbial method of producing inositol Microbial method of producing inositol

1992-07-21

1994-03-22

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435942, C12P 702

Patent

active

052963640

ABSTRACT:
A method for increasing production of inositol from a nutrient medium is disclosed. The wild strain of Saccharonyces cerrevisae was modified by deleting the OPI1 gene locus. The modified strain was fermented in various media, both artificial and natural, to increase the production of inositol from the medium. Using a balanced synthetic nutrient medium, a glucose feed rate of 100 g/l and adding ammonium sulfate as a nitrogen source gave an almost 10-fold increase in inositol production, compared to production under more typical conditions. Using almond hull juice as a feedstock, batch conditions showed no measureable increase in inositol production but continuous fermentation yielded almost a 50% increase in inositol concentration.

REFERENCES:
patent: 2615053 (1952-10-01), Artz et al.
patent: 5064762 (1991-11-01), Robinowitz
White, Michael J., et al., "The OPI1 Gene of Saccharomyces cerevisiae, a Negative Regulator of Phospholipid Biosynthesis, (Encodes a Protein Containing Polyglutamine Tracts and a Leucine Zipper)," J. of Biol. Chem. 266: 863-872 (1991).
Greenberg, Miriam L., "Regulatory Mutations of Inositol Biosynthesis in Yeast: [Isolation of Inositol Excreting Mutants]," Genetics 100: 19-33 (1982).
Bailey, J., et al., "Biochemical Engineering Fundamentals," pp. 579-580, McGraw-Hill (1986).
Morris, E. O., Chapter VI, "Yeast Growth", pp. 275-285, 317-321, 1958.
Greenberg et al., "Characterization of a Yeast Regulatory Mutant Constitute for Synthesis of Inositol-1-Phosphate Syntheses", Mol. Gen. Genet. 186, 157-163, 1982.

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