Microbial detection and quantification

Details Microbial detection and quantification Microbial detection and quantification

2005-04-28

2008-07-15

Weber, Jon P (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

Reexamination Certificate

active

07399608

ABSTRACT:
A method for semi-quantitatively or quantitatively detecting the presence of a microbe in a sample is provided. The method utilizes a test dye that undergoes a detectable color change in the presence of one or more microbes. For example, in one embodiment, the test dye is a solvatochromic dye (e.g., Reichardt's dye) that responds to differences in polarity between microbe components (e.g., cell membrane, cytoplasm, etc.) and the environment outside the cell. Alternatively, other mechanisms may be wholly or partially responsible for the interaction between the dye and the microbe, such as acid-base reactions, redox reactions, and so forth. Regardless, the color of the test dye may be compared to the color of a control dye, wherein the color of the control dye corresponds to a known microbe concentration.

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