Microbial degradation of trichloroethylene dichloroethylenes and

Details Microbial degradation of trichloroethylene dichloroethylenes and Microbial degradation of trichloroethylene dichloroethylenes and

1994-10-06

1996-08-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352523, 43525411, 4352625, 536 232, C12N 121, C12N 114, C12N 510, C12N 1553

Patent

active

055433170

ABSTRACT:
A novel bacterium named Pseudomonas cepacia PR1.sub.23 (formerly designated G4 5223 PR1) has the desirable property of constitutively degrading hazardous chemicals, for example trichloroethylene, 1,1-dichloroethylene, cis-1,2 dichloroethylene, trans-1,2-dichloroethylene, toluene, phenol, o-cresol, m-cresol, o-xylene, and benzene, to harmless chemical entities. This microbe, and mutants thereof which retain the constitutive degradation property of the parent, can be used in bioreactor and in situ processes for degrading hazardous chemical compounds. The nucleic acid sequences which encode the degradative peptides have also been isolated and sequenced. Cells transformed with the isolated nucleic acid also produce the peptides comprising the enzyme which can constitutively degrade these hazardous chemicals. The enzyme can be isolated from such microorganisms (those which naturally harbor the gene or those which are transformed with the gene) and applied to a sample having the hazardous chemical(s) or contaminant(s) in order to degrade the contaminant(s).

REFERENCES:
patent: 5171684 (1992-12-01), Yen et al.
Shields et al., Appl. Env. Microbiol. 61:1352-1356 (1995).

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