Microbial degradation of trichloroethylene

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Destruction of hazardous or toxic waste

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4351723, 4352523, 43525233, 43525234, 435264, 435189, 4353201, 435874, 210601, 935 59, C07C 126, C12N 1500, C12N 121, C12N 902, C12N 100, C02F 300, D06M 1600

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050791664

ABSTRACT:
This invention relates to a method for the microbial degradation of trichlorethylene by treating trichloroethylene with Pseudomonas mendocina KR-1 or Pseudomonas putida Y2101 or a microorganism host cell that contains a recombinant plasmid. The recombinant plasmid contains toluene monooxygenase genes from Pseudomonas mendocina KR-1. The microogranism host cell containing the recombinant plasmid must have been treated with an inducer of the toluene monooxygenase genes. The method may be applied to the treatment of loci of trichloroethylene chemical waste in water or soil. More particularly, the method may be applied to degrade trichloroethylene as it may be present as a pollutant or contaminant in water, in industrial effluents, in various land areas such as industrial sites, or in various laboratory or commercial installations.

REFERENCES:
patent: 4477570 (1984-10-01), Colaruotolo et al.
patent: 4520103 (1985-05-01), Ensley
Whitee, G. M. et al., Meeting for Am. Society for Microbiology, Mar. 3-7, 1985, Abstract Annu. Meeting.
Nelson et al., Appl. Environ. Micro., vol. 53, pp. 949-954 (1987).
Subramanian, V. et al., J. Biol. Chem., vol. 256, pp. 2723-2730 (1981).
Whitee, G. M., (1986) PhD. Thesis at The Univ. of Texas at Austin, Dissertation Abstracts International, p. 1861, vol. 47/05-B.

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