Microbial culture having catechol 1,2-oxygenase activity

Details Microbial culture having catechol 1,2-oxygenase activity Microbial culture having catechol 1,2-oxygenase activity

1986-01-14

1991-06-25

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435142, 435190, 435877, C12N 120, C12N 904, C12P 744

Patent

active

050266489

ABSTRACT:
This invention provides novel strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene or catechol to muconic acid quantitatively by the ortho (catechol 1,2-oxygenase) pathway.
Muconate lactonizing enzyme is not induced in the microorganism, thereby permitting the muconic acid to be produced and accumulated in a quantity greater than one gram of muconic acid per liter of bioconversion medium.

REFERENCES:
patent: 4355107 (1982-10-01), Maxwell
Gottschalk, "Bacterial Metabolism", Springer-Verlag, N.Y., 1979, pp. 126-131.
Vander Linden et al, "Microbial Oxidation of Hydrocarbons", Advances in Enzymology 27, pp. 506-516 (1965).
Ornston et al, "Isolation of Spontaneous Mutants of Pseudomonas Putida", Biochemical and Biophysical Research Communications 36(1), pp. 179-184 (1969).
Condon et al, "Cold Sensitive Mutation of Pseudomonas Putida Affecting Enzyme Synthesis at Low Temperatures", Journal of Bacteriology 94(6), pp. 1970-1981 (1967).
Kleinzeller et al, Chem. Listy 46, pp. 300-302 (1952), Chem. Abst. 47:4290a.
Hayaishi et al. Journal of the American Chemical Society 77, pp. 5450-5451 (1955).

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