Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Destruction of hazardous or toxic waste
Reexamination Certificate
2006-02-21
2006-02-21
Marx, Irene (Department: 1651)
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Destruction of hazardous or toxic waste
C435S042000, C435S252400, C435S244000, C424S093300
Reexamination Certificate
active
07001758
ABSTRACT:
A biodegradation process for the organophosphonate product of Sarin (O-isopropyl methylphosphonofluoridate) hydrolysis, i.e., isopropylmethylphosphonate (IMPA). This process provides a feasible biodegradation demilitarization alternative to Sarin incineration. Public opposition of nerve agent incineration is widespread, and alternative methods are sought to help the U.S. Army meet the 2007 demilitarization deadline imposed by the Chemical Weapons Convention. This process uses a two-step approach to IMPA biodegradation. In the first step, a concentrated IMPA solution is used as the sole nutritional carbon and phosphorus source for microbial cultures. The second step involves diluting the culture and adding an inexpensive carbon source to encourage bacterial phosphate assimilation. The biodegradation typically involves a consortium of microorganisms comprisingMethylobacterium radiotoleransGB21,Agrobacterium tumefaciensGB2GA,Klebsiella oxytocaGB2CS, GB272,Aureobacteriumsp. GB2 and three bacterial isolates belonging to the same species GB23, GB272, and GB292.
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Autenrieth, et al., In Situ and On-Site Bioremediation, Papers from the International In Situ and On-Site Bioremediation Symposium, 4th, New Orleans, Apr. 28-May 1, 1997, vol. 5, 49-54 Publisher: Battelle Press, Columbus, Ohio
Zhang, et al., Biotechnology and Bioengineering (1999), 64(2), 221-231.
DeFrank Joseph J.
Earley James P.
Fry Ilona J.
Biffoni Ulysses John
Marx Irene
The United States of America as represented by the Secretary of
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