Microbead reference standard and method of adjusting a flow cyto

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,...

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436 10, 356318, 356243, G01N 3100, G01J 330, G01J 102

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active

050734970

ABSTRACT:
A flow cytometer reference standard containing a highly uniform microbead population, wherein each microbead has the same multiple fluorescent labels as the other microbeads and as the samples to be measured. Target conditions are set for the sample after alignment of the flow cytometer. The microbead reference standard is used to determine peak channels (target channels) for each parameter. The target conditions may be reestablished for later uses of the flow cytometer with the same type of samples by using the microbead reference standard to adjust the flow cytometer so that the microbead peak channels fall in the originally determined target channels.

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