Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-09-07
1993-04-06
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 435 794, 435 795, 435805, 435810, 435967, 435969, 435970, 436518, 436528, 436531, 436169, 436170, 436805, 436808, 436810, 422 56, 422 57, 422 58, 422 60, G01N 33533
Patent
active
052003213
ABSTRACT:
A microassay card for a includes an upper layer containing wells for receiving a liquid sample. A second layer of the card, beneath the first layer, includes a supporting surface bound to a reactive species. A third layer includes a superabsorbent support impregnated with an indicator. Typically, the indicator is a substrate for an enzyme, such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme upon the substrate to cause a spectral change in the absorbent layer. By selecting the structure of the first and second layers, the card can be formatted for a displacement assay or a competitive assay. The microassay card of the present invention is particularly useful for drug testing.
REFERENCES:
patent: 4299916 (1981-11-01), Litman et al.
patent: 4391904 (1983-07-01), Litman et al.
patent: 4818677 (1989-04-01), Hay-Kaufman et al.
patent: 4826759 (1989-05-01), Guire et al.
patent: 4916056 (1990-04-01), Brown et al.
patent: 4920046 (1990-04-01), McFarland et al.
patent: 5006464 (1991-04-01), Chu et al.
Curme, et al. "Multilayer Film Elements for Clinical Analysis: General Copts" printed in Clin. Chem. 24/8, 1335-1342 (1978).
Zuk et al. "Enzyme Immunochromatography-A Quantitative Immunoasay Requiring No Instrumentation", printed in Clin. Chem 31/7, 1144-1150 (1985).
Ramp.TM. Urine hcG Assay brochure (1986) RMP002 30M 0186.
Granite Diagnostics, Inc. "Description of the E-Z Screen Test System" Brochure Oct. 7, 1985.
Grenner et al. "Multilayer Fluorescent Immunoassay Technique" reprinted in Clinical Chemistry, 34, 1865-1868, (1989).
Kidwell "Superabsorbent Polymers-Media for the Enzymatic Detection of Ethyl Alcohol in Urine", printed in Analytical Biochemistry, 182, 257-261 (1989).
Ceperley Mary E.
Edelberg Barry A.
McDonnell Thomas E.
Spiegel Carol A.
The United States of America as represented by the Secretary of
LandOfFree
Microassay on a card does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microassay on a card, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microassay on a card will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-535900