Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-08-26
1994-11-29
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 795, 435805, 435810, 435969, 435970, 436518, 436531, 436169, 436170, 42 56, 42 57, 42 58, 42 60, G01N 3353, G01N 33543
Patent
active
053690079
ABSTRACT:
A microassay card includes an upper layer containing wells for receiving a liquid sample. A second layer of the card, beneath the first layer, includes a supporting surface bound to a reactive species. A third layer includes a superabsorbent support impregnated with an indicator. Typically, the indicator is a substrate for an enzyme, such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme upon the substrate to cause a spectral change in the absorbent layer. By selecting the structure of the first and second layers, the card can be formatted for a displacement assay or a competitive assay. The microassay card of the present invention is particularly useful for drug testing.
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Edelberg Barry A.
McDonnell Thomas E.
Scheiner Toni R.
The United States of America as represented by the Secretary of
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