Microassay for detection of DNA and RNA

Details Microassay for detection of DNA and RNA Microassay for detection of DNA and RNA

1985-01-25

1991-02-26

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 935 77, 935 78, C12Q 168

Patent

active

049961446

ABSTRACT:
A novel hybridization diagnostic technique is involved employing micromanipulation of cells for detection of DNA or RNA in the cells. Cellular suspensions are employed, the DNA or RNA purified by microextraction using liquid media having differential solubilization properties, whereby the nucleic acid is retained in an aqueous microdrop. The nucleic acid in the microdrop may then be assayed with a nucleic acid probe in accordance with conventional ways.

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Scalenghe, F., et al. Chromosoma (Berl.), 82:205-216 (1981).
Collins, P. L., et al., Journ. Virol., 28, No. 1:324-336, (1978).

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