Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-11-27
2010-12-07
Kapushoc, Stephen (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S287200, C536S023100, C536S025300, C536S025310
Reexamination Certificate
active
07846660
ABSTRACT:
The present invention is a method for synthesizing microarrays having different oligonucleotides present within one feature area of the array. The method utilizes the techniques common to microarray synthesis, but limits the duration in which selected feature areas on the array are initially dosed with light so as to only deprotect a calculated ratio of the compounds forming the array's binding layer. The compounds initially deprotected are capped with a non-photosensitive protecting group, such as di-methoxy-trityl, to inhibit their involvement in the synthesis of a first group of DNA strands built onto the array. Once the first group of DNA strands have been synthesized, the original deprotected group may then be further processed to build one or more groups of DNA strands in the same feature area as the first group of DNA strands. The present invention also includes microarrays manufactured using the method.
REFERENCES:
patent: 5959098 (1999-09-01), Goldberg et al.
patent: 6426184 (2002-07-01), Gao et al.
patent: WO 9509248 (1995-04-01), None
patent: WO00/11223 (2000-03-01), None
Beier et al, Synthesis of photolabile 5′-O-phosparamidites for photolithographic production of microarrays of inversely oriented oligonucleotides, 2001, Helvetic Chimica Acta, 84, 2089-2095.
Albert et al., “Light-directed 5′—3′ synthesis of complex oligonucleotide microarray.” Nucleic Acids Research, 31:e35, 2003.
Albert Thomas
Green Roland
Norton Jason
Bhat Narayan K
Kapushoc Stephen
Quarles & Brady LLP
Roche Nimblegen, Inc.
LandOfFree
Microarrays having multiple oligonucleotides in single array... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microarrays having multiple oligonucleotides in single array..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microarrays having multiple oligonucleotides in single array... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4236434