Microarray synthesis and assembly of gene-length...

Details Microarray synthesis and assembly of gene-length... Microarray synthesis and assembly of gene-length...

2008-01-29

2008-01-29

Shibuya, Mark L. (Department: 1639)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S041000, C435S091100, C435S091200

Reexamination Certificate

active

07323320

ABSTRACT:
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

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