Micro-organisms with glycosylation modulating action of...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of...

Reexamination Certificate

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C424S093400, C424S093100

Reexamination Certificate

active

07008785

ABSTRACT:
The invention concerns micro-organism strains, in particular of lactic acid bacteria, having a glycosylation modulating effect of intestinal cell surface. The invention also concerns a method for selecting micro-organism strains, in particular of lactic acid bacteria, which consists in measuring the average fluorescence intensity variation of HT29-MTX cells incubated in the presence of a lectin coupled with a fluorochrome after being in contact with the supernatant of the strain concerned. Said lactic acid bacteria strains can be used, optionally in the form of their active fraction, for preparing food compositions or medicines or food supplements, modulating glycosylation of glycoproteins of intestinal epithelial cells.

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patent: 99/29833 (1999-06-01), None
XP-001001545 Abstract of Mack DR et al. “Modulation in the expression of intestinal MUC3 mucin by non-pathogenic probiotic microbes”.
Bry Lynn et al., “A Model of Host-Microbial Interactions in an Open Mammalian Ecosystem”, Science vol. 273, pp. 1380-1383, Sep. 6, 1996 XP-000993528.
Hooper Lora V. et al., “Host-microbial symbiosis in the mammalian intestine: exploring an internal ecosystem” BioEssays 20.4, Apr. 1998, XP-001003006.
Lesuffleur et al., “Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells”, 1990, Cancer Res., 50:6334-6343.

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