Micro-isolator steam sterilization cycle and apparatus

Chemical apparatus and process disinfecting – deodorizing – preser – Process disinfecting – preserving – deodorizing – or sterilizing – Using direct contact steam to disinfect or sterilize

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Details

422 33, 422110, 422112, 119 18, A61L 206, G05D 1600

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active

054298008

ABSTRACT:
A particularly useful method is disclosed for sterilizing micro-isolators in which a sipper bottle is contained. In the present invention, sterilization is effected by using a microprocessor control to regulate the pressure within a sterilization chamber in response to temperature conditions. Pressure within the chamber is regulated so that as temperature is raised and lowered, the boiling point of water in the sipper bottle is never exceeded. Prior to sterilization, the chamber is evacuated of air to permit effective distribution of steam. Subsequent to sterilization, the chamber is again evacuated in order to evaporate condensed steam that covers the micro-isolator and its contents. During each of these evacuations, the microprocessor control regulates the rate of vacuum draw down so that the boiling point of water in the sipper bottle is again not exceeded. This method permits micro-isolators to be sterilized with sipper bottles full of water, without losing a significant volume of water. The method of the present invention thus provides micro-isolators that are ready to use, including the drying of the micro-isolator, complete with bedding and food.

REFERENCES:
patent: 4108601 (1978-08-01), Wolff
patent: 4166096 (1979-08-01), Gillis
patent: 4238447 (1980-12-01), Wolff
patent: 4971764 (1990-11-01), Albright
"Validation of Steam Sterilization Cycles", PDA Technical Monograph No. 1, Parenteral Drug Association Research Committee and Task Groups, 1978.

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