Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-11-14
1999-12-14
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 41, 435182, 435243, 435325, 435382, 4352891, 4352971, 4352974, 435400, C12Q 102, C12N 500, C12M 100, C12M 300
Patent
active
060015853
ABSTRACT:
A "micro" hollow fiber bioreactor and method of use are provided for use in screening different cell lines and process conditions. The bioreactor includes the use of an oxygen permeable (e.g., silicone rubber) tube sealably containing a hollow fiber bundle, in order to create an extracapillary space to provide a medium reservoir and an intracapillary space for the growth of cells. The bioreactor avoids the need for oxygen or medium pumps or supply systems, and permits multiple cell lines, and/or multiple conditions to be evaluated simultaneously. Preferably, the tube has an oxygen permeability of between about 100.times.10.sup.-10 to about 10,000.times.10.sup.-10 (cc-mm/sec-cm.sup.2 -cm Hg), the extracapillary space provides a medium reservoir of about 1 ml to about 100 ml, the intracapillary space provides a cell culture volume of about 0.1 ml to about 1 ml, the hollow fibers have a molecular weight cut off from about 1 kD to about 1,000 kD and a pore size of from about 0.01 microns to about 5 microns, and the tube contains about 1 to about 1000 hollow fibers.
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Cellex Biosciences, Inc.
Naff David M.
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