Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1995-06-07
1999-01-12
Chambers, Jasemine C.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
4351723, 435 691, 435 701, 435325, 4353201, 536 234, 536 241, 424 921, C12N 500, C12N 1500, C12N 1509
Patent
active
058593101
ABSTRACT:
Transgenic mice carrying two transgenes, the first coding for a transactivator fusion protein comprising a tet repressor and a polypeptide which directly or indirectly activates transcription of a tet operator-linked gene in eucaryotic cells, and the second comprising a gene operably linked to a minimal promotor operably linked to at least one tet operator sequence, are disclosed. Isolated DNA molecules (e.g., targeting vectors) for integrating a polynucleotide sequence encoding a transactivator of the invention at a predetermined location within a second target DNA molecule by homologous recombination are also disclosed. Transgenic mice having the DNA molecules of the invention integrated at a predetermined location in a chromosome by homologous recombination are also encompassed by the invention. Methods to regulate the expression of a tet operator linked-gene of interest by administering tetracycline or a tetracycline analogue to a mouse of the invention are also disclosed. The regulatory system of the invention allows for conditional inactivation or modulation of expression of a gene of interest in a host cell or mouse.
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Bujard Hermann
Gossen Manfred
Salfeld Jochen G.
Voss Jeffrey W.
BASF - Aktiengesellschaft
Chambers Jasemine C.
DeConti, Jr. Giulio A.
Kara Catherine J.
Schmuck Jill D.
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