Multicellular living organisms and unmodified parts thereof and – Nonhuman animal
Patent
1995-01-26
1998-12-15
Crouch, Deborah
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
800DIG1, 800DIG2, 4351723, 4353201, 435354, C12N 500, C12N 1500
Patent
active
058499911
ABSTRACT:
Human pre-formed xenoantibodies play an important role in the hyperacute rejection response in human xenotransplantation. Disclosed are materials and methods for removing or neutralizing such antibodies. Also disclosed are materials and methods for reducing or eliminating the epitopes in the donor organs that are recognized by such antibodies. Such epitopes are formed as the result of activity by the enzyme .alpha.-1,3 galactosyltransferase. The porcine gene encoding .alpha.-1,3 galactosyltransferase is disclosed, as are materials and methods for inactivating ("knocking out") the .alpha.-1,3 galactosyltransferase gene in mammalian cells and embryos. Included are nucleic acid constructs useful for inactivating the .alpha.-1,3 galactosyltransferase gene in a target cell. Also disclosed is a novel leukemia inhibitory factor (T-LIF) that is useful for maintenance of embryonic stem cells and primordial germ cells in culture.
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Crawford Robert J.
d'Apice Anthony J. F.
Pearse Martin J.
Rathjen Peter D.
Robins Allan J.
Bresatch Limited
Crouch Deborah
St. Vincent's Hospital
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