Methylotroph cloning vehicle

Details Methylotroph cloning vehicle Methylotroph cloning vehicle

1987-11-13

1989-04-25

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 435320, 935 26, 935 29, 935 72, 536 27, C12N 120, C12N 1500

Patent

active

048247863

ABSTRACT:
A cloning vehicle comprising: a replication determinant effective for replicating the vehicle in a non-C.sub.1 -utilizing host and in a C.sub.1 -utilizing host; DNA effective to allow the vehicle to be mobilized from the non-C.sub.1 -utilizing host to the C.sub.1 -utilizing host; DNA providing resistance to two antibiotics to which the wild-type C.sub.1 -utilizing host is susceptible, each of the antibiotic resistance markers having a recognition site for a restriction endonuclease; a cos site; and a means for preventing replication in the C.sub.1 -utilizing host. The vehicle is used for complementation mapping as follows. DNA comprising a gene from the C.sub.1 -utilizing organism is inserted at the restriction nuclease recognition site, inactivating the antibiotic resistance marker at that site. The vehicle can then be used to form a cosmid structure to infect the non-C.sub.1 -utilizing (e.g., E. coli) host, and then conjugated with a selected C.sub.1 -utilizing mutant. Resistance to the other antibiotic by the mutant is a marker of the conjugation. Other phenotypical changes in the mutant, e.g., loss of an auxotrophic trait, is attributed to the C.sub.1 gene. The vector is also used to inactivate genes whose protein products catalyze side reactions that divert compounds from a biosynthetic pathway to a desired product, thereby producing an organism that makes the desired product in higher yields.

REFERENCES:
patent: 3663370 (1972-05-01), Kono
patent: 3907637 (1975-09-01), Nakayama
patent: 3963572 (1976-06-01), Gatenbeck
Haber et al. (1983), Science, 221:1147-1153.
Ditta et al. (1980), PNAS USA, 77:7347.
Friedman et al. (1982), Gene, 18:289.
Knauf et al. (1982), Plasmid, 8:45.
Holloway (1981), Microbial Growth on C--1 Compounds, H. Dalton Ed., London, pp. 317-324.
Rieb et al. (1980), Genet. Res., 36:99.
Bagdasarian et al. (1981), Gene, 16:237.
Gautier et al. (1980), Mol. Gen. Genet., 178:375.
Moore et al. (1983), J. Gen. Microbiol., 129:785-799.
Tatra et al. (1983), J. Gen. Microbiol., 129:2629-2632.
O'Connor et al. (1978), J. Gen. Microbiol., 104:105-111.
Warner et al. (1980), FEMS Microbiol. Letters, 7:181-185.
Jeyaseelan et al. (1979), FEMS Microbiol. Letters, 6:87-89.
Haber, 1984, "Plasmid DNA from an Obligate Methanetroph: Physical and Conjugative Characteristics", Ph.D. Dissertation, Univ. Wisc. Madison, pp. 128-156.
Gowrishankar et al. 1982, "Regulation of Phenylalanine Biosynthesis in E. Coli K--12 . . . ", J. Bact., v 150(3), pp. 1130-1137.
Toukdarian et al. (1984), Journal of Bacteriology, 157(3):979-983.
Suzuki et al. (1977), J. Ferment. Technol., 55(s):466-475.
Allen et al. (1984), Microbial Growth on C1 Compounds, Proc. 4th Int'l. Symp. Crawford, Ed.

Affiliated with

Also associated with

Rating

Methylotroph cloning vehicle does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methylotroph cloning vehicle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methylotroph cloning vehicle will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1195579