Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2007-05-29
2007-05-29
Steadman, David J. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S115000, C435S252100, C435S252300, C435S194000, C435S232000, C536S023100, C536S023200, C536S023700
Reexamination Certificate
active
09926299
ABSTRACT:
An L-amino acid is produced by culturing aMethylophilusbacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, aMethylophilusbacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or aMethylophilusbacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.
REFERENCES:
patent: 4824786 (1989-04-01), Hanson et al.
patent: 5939307 (1999-08-01), Wang et al.
patent: 6040160 (2000-03-01), Kojima et al.
patent: 6303381 (2001-10-01), Gunji et al.
patent: 6350596 (2002-02-01), Iomantas et al.
patent: 35831 (1981-09-01), None
patent: 37273 (1981-10-01), None
patent: 0435132 (1991-07-01), None
patent: 0 857 784 (1998-08-01), None
patent: 53-34987 (1978-03-01), None
patent: 1-235595 (1989-09-01), None
patent: 87 02984 (1987-05-01), None
patent: WO 95/16042 (1995-06-01), None
patent: 96/41871 (1996-12-01), None
Branden et al. “Introduction to Protein Structure”, Garland Publishing Inc., New York, 1991, p. 247.
Witkowski et al. (1999) Biochemistry 38:11643-11650.
Voet et al. “Biochemistry, 2nd Ed.,” John Wiley and Sons, Inc., New York, 1995, pp. 762-763.
“Current Protocols in Molecular Biology” Ausubel et al., John Wiley and Sons, Inc., New York, 1993, Unit 2.9A, pp. 1-2 and Unit 2.10, pp. 1-16.
Proc Natl Acad Sci (1982) 79:4256-4259, De Maeyer et al.
J. D. Cirillo, et al., Molecular Microbiology, vol. 11, No. 4, pp. 629-639, XP-002907355, “Isolation and Characterization of the Aspartokinase and Aspartate Semialdehyde Dehydrogenase Operon from Mycobacteria”, 1994.
Database EMBL Online !, Database Accession No. Z17372, pp. 1-3, XP-002244623, Aug. 9, 1994.
Database EMBL Online !, Database Accession No. P41403, pp. 1-2, XP-002244624, Nov. 1, 1995.
Derwent Abstracts, AN 1993-297465, XP-002244625, JP 05-207866, Aug. 20, 1993.
Kim, et al., “Creating auxotrophic mutants inMethylophilus methylotrophusAS1 by combining electroporation and chemical mutagenesis”, Applied Microbiology and Biotechnology, vol. 48, No. 1, (1997), pp. 105-108.
J.D. Windass et al.: “Improved converstion of methanol to single-cell protein byMethylophilus methylorophus” NATURE, vol. 287, pp. 396-401 Oct. 2, 1980.
Frederick J. Schendel, et al.: “Cloning and Nucleotide Sequence of the Gene Coding for Aspartokinase II from a Thermophilic MethylotrophicBacillussp.” Applied and En-Vironmental Microbiology, vol. 58, No. 9, pp. 2806-2814 Sep. 1992.
Tung T. Hoang, et al.: “Molecular genetic analysis of the region containing the essentialPseudomonas aeruginosa asdgene encoding aspartate-beta-semialdehyde dehydrogenase” MICROBIOLOGY, vol. 143, Part 3, pp. 899-907 Mar. 1997.
Yoshihiro Yamamoto et al.: “Construction of a Contiguous 874-kb Sequence of theEscherichia coli-K12 Genome corresponding to 50.5-68.8 min on the Linkage Map and Analysis of Its Sequence Features” DNA Research, vol. 4, No. 2, pp. 91-113 Apr. 28, 1997.
S. Bonnassie et al.: “Nucleotide sequence of the dapA gene fromCorynebacterium glutamicum” Nucleic Acids Research, vol. 18, No. 21, p. 6421 Nov. 11, 1990.
J. Bouvier et al.: “Nucleotide Sequence and Expression of theEscherichia coli dapB Gene” The Journal of Biological Chemistry, vol. 259, No. 23, pp. 14829-14834 Dec. 10, 1984.
Linda C. Dekkers et al. “A site-specific recombinase is required for competitive root colonization byPseudomonas fluorescensWCS365” Proceedings of the National Academy of Sciences, USA, vol. 95, No. 12, pp. 7051-7056 Jun. 9, 1998.
P. Kerney et al: “Regulations and routes of biosynthesis of serin and arginine inMethylophilus methylotrophusASI” FEMS Microbiology Letters, vol. 42, Nos. 2-3, pp. 109-112 Jul. 1987.
Office Action by the Chinese Patent Office for Chinese Patent Appl. No. 00806019.3 (Jan. 16, 2004).
Gunji Yoshiya
Miyata Yuri
Oba Manami
Shimaoka Megumi
Sugimoto Shinichi
Cermak Shelly Guest
Cermak & Kenealy LLP
Steadman David J.
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