Methylene blue and toluidene blue mediated fluorescence diagnosi

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Diagnostic or test agent produces in vivo fluorescence

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424 97, 424 98, 436172, 436800, A61B 1000, G01N 2176

Patent

active

060834870

ABSTRACT:
A method for differentiating between normal tissue and tumor tissue within a tissue field. The method includes the application of a photosensitizer solution to the tissue field and the subsequent illumination and measurement of fluorescent intensity across the tissue field. The measurement of relative fluorescent intensity across the tissue field provides information relating to the existence or nonexistence of tumor tissue. Solution concentrations of methylene blue and or toluidene blue are utilized to provide the greatest differentiability between normal tissue and tumor tissue within the tissue field.

REFERENCES:
patent: 5372801 (1994-12-01), Malmros et al.
Gill, W.B. et al., Selective Surface Staining of Bladder Tumors by Intravesical Methylene Blue with Enhanced Endoscopic Identification, Cancer, 53, 2724-2727, 1984.
Peng, Q. et al., Biodistribution of a methylene blue derivative in tumor and normal tissues of rats, Jour. Photochem. Photobiol, B: Biol., 20, 63-71, 1993.

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