Methods using novel chimeric transforming growth factor-.beta.1/

Details Methods using novel chimeric transforming growth factor-.beta.1/ Methods using novel chimeric transforming growth factor-.beta.1/

1991-03-14

1994-04-19

Hill, Jr., Robert J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

530399, 930120, C07K 1300

Patent

active

053045416

ABSTRACT:
The present invention relates to the production of large quantities of a novel chimeric TGF-.beta., termed TGF-.beta.1/.beta.2, by eucaryotic host cells transfected with recombinant DNA vectors containing the TGF-.beta.1/.beta.2 precursor coding sequence controlled by expression regulatory elements. Simian TGF-.beta.1 cDNA (Sharples et al., 1987, DNA 6:239-244) was modified so that the nucleotides encoding amino acid residue numbers 9-13, 17, 19, 25 and 26 of the mature TGF-.beta.1 sequence were hanged to the nucleotides encoding the corresponding amino acids of the mature TGF-.beta.2 structure. Simian codon usage was maintained. The chimeric TGF-.beta.1/.beta.2 of the invention induces effects on the proliferation of vascular endothelial cells equivalent to those induced by TGF-.beta.1.

REFERENCES:
Gentry et al., Mol. and Cell Biol., 7(10):3418-27 (1987).
Madisen et al., DNA 7(1):1-8 (1988).
Meister et al., J. Gen. Virol., 67:1633-1643 (1986).
Keller et al., J. Exp. Med., 168:737-750 (1988).
Postlewaite et al., J. of Exp. Med., 165:251-56 (1987).

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