Methods to replicate DNA in vitro using PRD1-catalyzed DNA repli

Details Methods to replicate DNA in vitro using PRD1-catalyzed DNA repli Methods to replicate DNA in vitro using PRD1-catalyzed DNA repli

1994-03-09

1995-02-14

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 4351721, 536 231, 536 241, 935 1, 935 16, 935 17, C12Q 168, C12P 1934, C07H 1700, C12N 1500

Patent

active

053895312

ABSTRACT:
This invention relates to artificial DNAs which are useful in the fields of genetic engineering and medical science and to a method for DNA replication using said DNAs as the replication origin. In one embodiment, this invention provides single- or double-stranded, artificial DNAs having, at the 3' end, a DNA sequence represented by the following general formula:

REFERENCES:
Yoo et al., J. Mol. Biol., 218:779-789, 1991, (Mar. 20, 1991).
Savilahti et al., Gene 57 (1987) 121-130.
Yoo et al. J. Mol. Biol. 218: 779-789 '91 (Mar. 20, 1991).
Tamanoi et al. P.N.A.S. 80: 6446-6450 '83.
"Protein-Primed Replication of Bacteriophage PRD1 Genomie in Vitro", by Seung-Ku Yoo and Junetsu Ito, 170 Virology, pp. 442-449 (1989).
"Bacteriophage PRD1 DNA polymerase: Evolution of DNA polymerases", by Guhung Jung, Mark C. Leavitt, Jui-Cheng Hsieh, and Junetsu Ito, 84 Proc. Nat. Acad. Sci., U.S.A., pp. 8287-8291 (1987).
"Primary Structure of the DNA terminal protein of bacteriophage PRD1", by Jui-Cheng Hsieh, Guhung Jung, Mark C. Leavitt, and Junetsu Ito, 15 Nucl. Acids. Res., pp. 8999-9009.
"The complete nucleotide sequence of the left very early region of Escherichia coli bacteriophage PRD1 coding for the terminal protein and the DNA polymerase", 57 Gene, pp. 121-130 (1987).
"Molecular Cloning of Bacteriophage PRD1 Genomic Fragments", Mol. Gen. Genet., pp. 233-236 (1983).

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