Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2002-03-27
2004-09-21
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S071100, C435S069100, C435S325000
Reexamination Certificate
active
06794163
ABSTRACT:
TECHNICAL FIELD
The present invention relates to the field of circovirus and provides compositions and methods for culturing circovirus, in particular porcine circovirus. In particular, the present invention relates to methods for culturing porcine circovirus in mammalian cells expressing a mammalian adenovirus E1 gene function.
BACKGROUND ART
A family of viruses, named Circoviridae, found in a range of plant and animal species and commonly referred to as circoviruses, are characterized as round, non-enveloped virions with mean diameters from 17 to 23.5 nm containing circular, single-stranded deoxyribonucleic acid (ssDNA). The ssDNA genome of the circoviruses represent the smallest viral DNA replicons known. As disclosed in WO 99/45956, at least six viruses have been identified as members of the family according to The Sixth Report of the International Committee for the Taxonomy of Viruses (Lukert, P. D. et al. 1995
, The Circoviridae
, pp. 166-168. In F. A. Murphy, et al. (eds.) Virus Taxonomy, Sixth Report of the International Committee on Taxonomy of Viruses, Arch. Virol. 10 Suppl.).
Animal viruses included in the family are chicken anemia virus (CAV); beak and feather disease virus (BFDV); porcine circovirus (PCV); and pigeon circovirus. PCV was originally isolated in porcine kidney cell cultures. PCV replicates in the cell nucleus and produces large intranuclear inclusion bodies. See Murphy et al. (1999
, Circoviridae
p. 357-361, Veterinary Virology, 3rd ed. Academic Press, San Diego). There are currently two recognized types of PCV, PCV type 1 (PCV1) and PCV type 2 (PCV2). PCV1, isolated as a persistent contaminant of the continuous porcine kidney cell line PK-15 (ATCC CCL31), does not cause detectable cytopathic effects in cell culture and fails to produce clinical disease in pigs after experimental infection (see Allan G., 1995
, Vet. Microbiol
. 44: 49-64; Tischer, I. et al., 1982
, Nature
295:64-66; and Tischer, I. et al., 1986
, Arch. Virol
. 91:271-276). PCV2, in contrast to PCV1, is closely associated with post weaning multisystemic wasting syndrome (PMWS) in weanling pigs (see Allan G. et al., 1998
, Europe. J. Vet. Diagn. Investig
. 10:3-10; Ellis, J. et al., 1998
, Can. Vet. J
. 39:44-51 and Morozov, I. et al., 1998
, J. Clin. Microbiol
. 36:2535-2541). The nucleotide sequences for PCV1 are disclosed in Mankertz, A., et al. (1997
, J. Virol
. 71:2562-2566) and Meehan, B. M., et al. (1997
, J. Gen. Virol
. 78:221-227) and the nucleotide sequences for PCV2 are disclosed in Hamel, A. L. et al. (1998
, J. Virol
. 72:5262-5267); Mankertz, A. et al. (2000
, Virus Res
. 66:65-77) and Meehan, B. M. et al. (1998
, J. Gen. Virol
. 79:2171-2179). Strains of PCV2 are disclosed in WO 00/01409 and have been deposited at the European Collection of Cell Cultures, Centre for Applied Microbiology & Research, Porton Down, Salisbury, Wiltshire SP4 OJG, United Kingdom and include: accession No. V97100219; accession No. V9700218; accession No. V97100217; accession No. V98011608; and accession No. V98011609. WO 00/77216 also discloses PCV2.
Published studies to date on PCV2 used either tissue homogenate or cultured virus derived from field isolates. Tischer et al. (1987
, Arch Virol
. 96:39-57) report that porcine kidney cells are stimulated to entry to the S phase in the cell cycle by D-glucosamine treatment. However, the treatment must be performed with caution because D-glucosamine is toxic for cell culture (see, Allan et al., (2000).
J. Vet. Diagn. Investigation
. 12:3-14). There remains a need for methods for culturing circovirus, such as for example, PCV1 and PCV2, and other circoviruses, such that pure circovirus is obtained. Such methods would be advantageous, in particular for preparation of PCV2 antigens as vaccines directed against PMWS. The present invention addresses that need.
All patents and publications are hereby incorporated herein in their entirety.
DISCLOSURE OF THE INVENTION
The present invention provides methods for culturing mammalian circovirus comprising: a) obtaining mammalian cells expressing a mammalian adenovirus E1 function, wherein said cells are permissive for mammalian circovirus replication; b) introducing said mammalian circovirus genome, or a portion thereof capable of replication, into said mammalian cells; and c) culturing said mammalian cells under conditions suitable for replication of said mammalian circovirus. In some embodiments, the method further comprises recovering said circovirus from said cultured cells.
In some embodiments, the mammalian circovirus is porcine circovirus, such as for example, porcine circovirus 1 (PCV1) or porcine circovirus 2 (PCV2). In yet additional embodiments, the porcine circovirus comprises a chimeric nucleotide sequence. In other embodiments, the mammalian cells are of porcine origin. In yet other embodiments, the mammalian cells are porcine retina cells.
In other embodiments, the mammalian adenovirus E1 function is human adenovirus E1 function. In yet other embodiments, the mammalian adenovirus E1 function is porcine adenovirus E1 function. In further embodiments, the E1 function is E1A and/or E1B function. In yet further embodiments, the mammalian cell expressing the mammalian E1 function is stably transformed with mammalian E1 gene sequences. In other embodiments, the mammalian E1 gene sequence is heterologous to said mammalian cell.
The present invention also provides recombinant mammalian cells that express a mammalian adenovirus E1 function and comprise a mammalian circovirus genome, or a portion thereof capable of replication, and wherein said cells are permissive for the replication of said mammalian circovirus. In some embodiments, the mammalian circovirus is porcine circovirus, such as for example, porcine circovirus 1 (PCV1) or porcine circovirus 2 (PCV2). In yet additional embodiments, the porcine circovirus comprises a chimeric nucleotide sequence. In some embodiments, the adenovirus E1 function is human adenovirus E1 function. In other embodiments, the E1 function is porcine adenovirus E1 function. In other embodiments, the mammalian cell is of porcine origin. In further embodiments, the mammalian cell is a porcine retinal cell. In yet further embodiments, the mammalian cell expressing the mammalian E1 function is stably transformed with mammalian adenovirus E1 gene sequences. In other embodiments, the mammalian E1 gene sequence is heterologous to said mammalian cell.
The present invention also provides methods of preparing a recombinant mammalian cell expressing a mammalian adenovirus E1 function and comprising a mammalian circovirus genome comprising the steps of, a) obtaining a mammalian cell expressing a mammalian adenovirus E1 function; and b) introducing said mammalian circovirus genome, or a portion thereof capable of replication, into said mammalian cell. In additional embodiments, the method comprises the additional step of culturing the recombinant mammalian cell under conditions suitable for the replication of said mammalian circovirus. In further embodiments, the method comprises recovering said circovirus from said cultured cells. In some embodiments, the mammalian circovirus is porcine circovirus, such as for example, porcine circovirus 1 (PCV1) or porcine circovirus 2 (PCV2). In yet additional embodiments, the porcine circovirus comprises a chimeric nucleotide sequence. In further embodiments, the mammalian cells are of porcine origin. In yet further embodiments, the mammalian cells are porcine retina cells. In additional embodiments, the adenovirus E1 function is human adenovirus E1 function or porcine adenovirus E1 function. In yet further embodiments, the mammalian cell expressing the mammalian adenovirus E1 function is stably transformed with mammalian adenovirus E1 gene sequences. In other embodiments, the mammalian E1 gene sequence is heterologous to said mammalian cell.
REFERENCES:
patent: 6217883 (2001-04-01), Allan et al.
patent: 6287856 (2001-09-01), Poet et al.
patent: 6368601 (2002-04-01), Allan et al.
patent: 6391314 (2002-05-01), Allan et al.
patent: 6492343 (200
Babiuk Lorne A.
Liu Qiang
Tikoo Suresh K.
Willson Philip
Chen Stacy B.
Housel James
University of Saskatchewan
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