Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1992-03-11
1993-11-16
Parr, Margaret
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, C12P 1934, C12Q 168
Patent
active
052623114
ABSTRACT:
A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers, one being short with arbitrary sequence and another being either short with arbitrary sequence or being capable of hybridizing to the region near the mRNA polyA tail. The oligodeoxynucleotide that is capable of hybridizing to the region near the polyA tail is used as a primer for reverse transcription of the mRNA and the resultant cDNA is amplified with a polymerase using both oligodeoxynucleotides as a primer set.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Liang et al. Science 257:967-971 (1992).
Gilliland et al. in "PCR protocols: A guide to methods and application" pp. 60-69, 1990.
Freifelder, D. "Molecular Biology" pp. 402-404 1983.
Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Press, pp. 8.6-8.35 (1989).
Sargent, T. D., "Isolation of Differentially Expressed Genes", 1987, Methods in Enzymology, vol. 152, pp. 423-ff.
Lee, S. W., et al., "Positive Selection of Candidate Tumor-Suppressor Genes by Subtractive Hybridization", 1991, Proc. Natl. Acad. Sci. USA, vol. 88, pp. 2825-2829.
Walker, G. T., et al., "Isothermal In Vitro Amplification of DNA by a Restriction Enzyme/DNA Polymerase System", 1992, vol. 89, pp. 392-396.
Williams, J., et al., "DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Markers", 1990, Nucleic Acids Research, vol. 18, pp. 6531-6535.
Liang Peng
Pardee Arthur B.
Dana-Farber Cancer Institute Inc.
Parr Margaret
Tran Paul B.
LandOfFree
Methods to clone polyA mRNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods to clone polyA mRNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods to clone polyA mRNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-21732