Methods relating tosterol regulatory element binding proteins

Details Methods relating tosterol regulatory element binding proteins Methods relating tosterol regulatory element binding proteins

1996-06-14

1999-04-06

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435367, 435369, C12Q 168, G01N 3353

Patent

active

058916318

ABSTRACT:
A sterol regulatory element (SRE) binding protein (SREBP) which activates transcription from SREs, such as SRE-1 of the low intensity lipoprotein (LDL) receptor gene, is disclosed, as are DNA segments encoding SREBPs such as an SREBP-1 or SREBP-2. Also described are methods for using SREBP to promote SRE-mediated transcription and LDL receptor production in the presence of sterols, and screening assay for the identification of further agents with such properties. The SREBP and other agents may be used to reduce plasma cholesterol levels and to treat various medical problems associated with hypercholesterolemia.

REFERENCES:
patent: 4935363 (1990-06-01), Brown et al.
patent: 5215910 (1993-06-01), Brown et al.
patent: 5256545 (1993-10-01), Brown et al.
patent: 5498696 (1996-03-01), Briggs et al.
patent: 5527690 (1996-06-01), Goldstein et al.
Ayer et al., "Mad: A Heterodimeric Partner for Max That Antagonizes Myc Transcriptional Activity," Cell, 72:211-222, 1993.
Gil et al., "Purification of a Protein Doublet that Binds to Six TGG-Containing Sequences in the Promoter for Hamster 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase," J. Biol. Chem., 263(35):19009-19019, 1988.
Gil et al., "Multiple Genes Encode Nuclear Factor 1-Like Proteins that Bind to the Promoter for 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase," Proc. Natl. Acad. Sci. USA, 85:8963-8967, 1988.
Rajavashisth et al., "Identification of a Zinc Finger Protein that Binds to the Sterol Regulatory Element," Science, 245:640-643, 1989.
Stark et al., Common Double- and Single-Stranded DNA Binding actor for a Sterol Regulatory Element, Proc. Natl. Acad. Sci. USA, 89:2180-2184, 1992.
Briggs et al., "Nuclear Protein That Binds Sterol Regulatory Element of Low Density Lipoprotein Receptor Promoter," J. Biol. Chem., 268(19):14490-14496, 1993.
Hua et al., "SREBP-2, a Second Basic-Helix-Loop-Helix-Leucine Zipper Protein that Stimulates Transcription by Binding to a Sterol Regulatory Element," Proc. Natl. Acad. Sci. USA, 90:11603-1167, 1993.
Osborne, "Single Nucleotide Resolution of Sterol Regulatory Region in Promoter for 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase," J. Biol. Chem., 266(21): 13947-13951, 1991.
Wang et al., "Nuclear Protein That Binds Sterol Regulatory Element of LowDensity Lipoprotein Receptor Promoter," J. Biol. Chem., 268(19):14497-14504, 1993.
Yokoyama et al., "SREBP-1, a Basic-Helix-Loop-Helix-Leucine Zipper Protein That Controls Transcription of the Low Density Lipoprotein Receptor Gene," Cell, 75:187-197, 1993.
PCT Search Report, dated Nov. 28, 1994.
Agnani et al., "Antipeptide antibody against the human low-density-lipoprotein receptor," Biochem. J., 263:753-760, 1989.
Briggs et al., "Purufication and Biochemical Characterization of the Promoter-Specific Transcription Factor, Sp1," Science, 234:47-52, 1986.
Brown and Goldstein,"A Receptor-Mediated Pathway for Cholesterol Homeostasis," Science, 232:34-47, 1986.
Smith et al., "Identification of Nucleotides Responsible for Enhancer Activity of Sterol Regulatory Element in Low Density Lipoprotein Receptor Gene," The Journal of Biological Chemistry, 265(4):2306-2310, 1990.

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