Methods, reagents and test kits for determination of subpopulati

Details Methods, reagents and test kits for determination of subpopulati Methods, reagents and test kits for determination of subpopulati

1989-05-01

1993-10-26

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

436504, 436518, 436526, 436536, 436538, 436 71, 436172, 436173, 435 72, 435 724, 435 725, 435 732, 435 792, C12Q 170

Patent

active

052565327

ABSTRACT:
Analytes, having a characteristic determinant that selectively interacts with a specific binding substance, are determined by coupling a reporter substance to the analyte, or to the specific binding substance, causing complex formation between the analyte and the specific binding substance in a test medium, separating the complexes thus formed from the test medium, and determining the presence or quantity of the analyte of interest by detecting the occurrence of the reporter substance in the complexes or the separated test medium. The determination is preferably performed on biomembrane-containing entities, such as cell subpopulations and/or subsets thereof, the reporter substance being stably associated with the lipid component of the biomembrane. Reagents and test kits are disclosed for performing the analyte determination.

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