Methods of using non-plant insecticidal lipase encoding...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S278000, C800S298000, C800S295000, C800S312000, C800S320000, C800S317000, C800S306000, C800S322000, C800S314000, C800S301000, C435S468000, C435S419000

Reexamination Certificate

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07491869

ABSTRACT:
Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

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patent: 1 130 100 (2001-09-01), None
Schmitt, J., et al., Blocking the Tunnel: Engineering ofCandida rugosaLipase Mutants with Short Chain Length Specificity, Protein Engineering, 2002, 15(7): 595-601.
Strickland, J. et al., Inhibition of Diabrotica Larval Growth by Patatin, the Lipid Acyl Hydrolase from Potato Tubers, Plant Physiol, 1995, 109:667-674.
Beer et al., Cloning, expression, characterization and role of th eleader sequence of a lipase fromRhizopus oryzae, Bioch. et Biophys. Acta, 1998, 1399: 173-180.
Berto et al., Occurrence of a lipae in spores ofAlternaria brassicicolawith a crucial role in the infection of cauliflower leaves, FEMS Microbiology Letters, 1999, 180: 183-189.

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