Methods of using molecular constructs for detection of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S004000, C536S023100, C536S024300

Reexamination Certificate

active

07320864

ABSTRACT:
The present invention relates to molecular constructs and methods of their use in detecting biochemical reactions. In particular, the invention relates to a molecular construct having a capture portion and a substrate portion, where the capture portion isolates the construct from a sample medium, and the substrate portion enables the construct to be acted upon and undergo a physical change which can be detected and measured. These molecular constructs may be used in diagnostic assays, genetic screening for potential risks of certain diseases in individuals, and drug discovery and toxicogenomics, using high throughput screening of compounds.

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Koch et al., “PNA-Peptide Chimerae,” Tetrahedron Letters 36 : 6933-6936 (1995).
Niemeyer et al., Oligonucleotide-directed self-assembly of proteins: semisynthetic DNA—streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates, Nucleic Acids Research, vol. 22: 5530-5539 (1994).
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Partial European Search Report, Mar. 19, 2004.

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