Methods of using functional 30S subunits

Details Methods of using functional 30S subunits Methods of using functional 30S subunits

2005-03-30

2008-12-02

Kam, Chih-Min (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C536S023100, C530S300000, C530S350000

Reexamination Certificate

active

07459290

ABSTRACT:
A method of preparing a peptide in vitro in a defined translation mixture is provided. The method includes providing a defined translation reaction mixture comprising isolated and/or purified 30S subunits comprising small subunit ribosomal proteins and 16S rRNA, 50S subunits comprising large subunit ribosomal proteins, 5S rRNA and 23S rRNA, charged tRNAs for at least one amino acid, an isolated RNA template for translation with at least one codon corresponding to the anticodon in the charged tRNAs, and one or more isolated translation factors.

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