Methods of using FET labeled oligonucleotides that include a...

Details Methods of using FET labeled oligonucleotides that include a... Methods of using FET labeled oligonucleotides that include a...

2007-01-09

2007-01-09

Horlick, Kenneth R. (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C435S091100, C435S091200, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

10222943

ABSTRACT:
Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.

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EPO Supplemental Search Report, Mailed Mar. 14, 2006.

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