Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1995-02-09
1997-05-13
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435135, 435220, 435221, 435222, C12P 2106, C12P 762, C12N 954, C12N 956
Patent
active
056291736
ABSTRACT:
The invention relates to serine protease variants derived from precursor serine proteases via recombinant and/or chemical methods to form protease variants having improved peptide ligase activity. The invention also includes novel ligation substrates which in combination with the serine protease variants and a second ligation substrate are capable of forming a ligation product. The invention also relates to methods for forming such ligation products and the products formed thereby.
REFERENCES:
patent: 4760025 (1988-07-01), Estell et al.
patent: 5155033 (1992-10-01), Estell et al.
Caldwell, R., et al., "Analysis of the Catalytic Importance of a Helix Dipole in Subtilisin Using Site-Directed Mutagenesis", American Chemical Society Abstract MBTD-73, Sep. 10-15 1989.
Carter, P., et al., "Dissecting the Catalytic Triad of a Serine Protease", Nature, 332(7):564-568 (1988).
Abrahmsen, L., et al., "Engineering Subtilisin and Its Substrates for Efficient Ligation of Peptide Bonds in Aqueous Solution", Biochemistry, 30:4151-4159 (1991).
Abrahmsen Lars
Burnier John
Jackson David Y.
Wells James A.
Genentech Inc.
Hendricks Keith D.
Trecartin Richard F.
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