Methods of use of human manganese superoxide dismutase

Details Methods of use of human manganese superoxide dismutase Methods of use of human manganese superoxide dismutase

1995-01-09

1996-07-30

Patterson, Jr., Charles L.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Oxidoreductases

435184, A61K 3750

Patent

active

055409113

ABSTRACT:
This invention provides plasmids for expression of human superoxide dismutase of analogs thereof. The invention further provides a method of producing enzymatically active human MnSOD or an analog thereof by introducing plasmids for expression of human MnSOD into procaryotic or eucaryotic cells and growing the resulting cells under suitable conditions, including conditions wherein the production medium is supplemented with Mn.sup.++. The invention additionally provides a method of recovering purified enzymatically active MnSOD from procaryotic and eucaryotic cells. Human MnSOD or analogs thereof may be used to reduce reperfusion injury, prolong the survival time of isolated organs or treat inflammations or bronchial pulmonary dysplasia.

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