Methods of tissue engineering

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of detaching cells – digesting tissue or establishing...

Reexamination Certificate

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C435S395000, C435S402000, C524S001000

Reexamination Certificate

active

08071379

ABSTRACT:
An improved substrate for growing mono-layers of adherent-type cells and methods of growing tissue structures, ex vivo. The improved substrate, which comprises a silicon substrate coated with a photo cleavable polymer, releases adherent cells non-enzymatically. Also disclosed are methods for assembling complex layers of cells of various types.

REFERENCES:
Nakanishi et al. (2008) Analytical Sciences, vol. 24, 67-72.
Kumar et al. (2005) The 229th ACS National Meeting in San Diego, CA, Mar. 13-17, 2005, BIOT 422, abstract.
Iwasaki et al. (2007) Colloids and Surfaces B: Biointerfaces, vol. 57, 226-236.
Riess et al. (2003) Prog. Polym. Sci., vol. 28, 1107-1170.
Chan-Park et al. (2004) Macromol. Biosci., vol. 4, 665-673.
Russell (2002) Science, vol. 297, 964-967.
Levrand, et al.; “Light induced controlled release of fragrances by Norrish type II photofragmentation of alkyl phenyl ketones;” PPS Paper; www.rsc.org/pps; Oct. 2002.

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