Methods of synthesizing oligonucleotides with random codons

Details Methods of synthesizing oligonucleotides with random codons Methods of synthesizing oligonucleotides with random codons

1995-02-27

1996-06-04

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, 536 2531, 536 2533, 536 2534, C07H 2102, C07H 2104

Patent

active

055233880

ABSTRACT:
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.

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Blake, R. D. et al. "Tuplet Analysis of Sequences." Federation Proceedings--Abstracts. Indexes #2958. (1983).
Adams, Roger L. et al. The Biochemistry of the Nucleic Acids. Publisher: Chapman and Hall, London and New York. pp. 11-12 (1986).

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