Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Reexamination Certificate
2011-04-19
2011-04-19
Joike, Michele K (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
C435S254200, C435S006120
Reexamination Certificate
active
07927863
ABSTRACT:
Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
REFERENCES:
patent: 4775622 (1988-10-01), Hitzeman et al.
patent: 5324660 (1994-06-01), Gleeson et al.
patent: 6204023 (2001-03-01), Robinson et al.
patent: 6258559 (2001-07-01), Zamost
patent: 6306625 (2001-10-01), Jacobs et al.
patent: WO 00/23579 (2000-04-01), None
patent: WO 03/018761 (2003-03-01), None
patent: WO 2003/029456 (2003-04-01), None
Macauley-Patrick et al. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270, 2005).
Blaise L. et al.: “Construction and diversification of yeast cell surface displayed libraries by yeast mating: application to the affinity maturation of Fab antibody fragments” Gene, vol. 342, Sep. 2004, pp. 211-218.
Non-Final Office Action mailed Mar. 18, 2009 in co-pending U.S. Appl. No. 10/577,074.
Final Office Action mailed Jan. 5, 2010 in co-pending U.S. Appl. No. 10/577,074.
Advisory Action in U.S. Appl. No. 10/577,074 mailed May 12, 2010.
Cregg James M.
Latham John
Litton Mark
Schatzman Randall
Tolstorukov Ilya I.
Alder BioPharmaceuticals, Inc.
Hunton & Williams LLP
Joike Michele K
Keck Graduate Institute
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