Methods of sequencing and detection using energy transfer labels

Details Methods of sequencing and detection using energy transfer labels Methods of sequencing and detection using energy transfer labels

1998-10-01

2000-11-21

Riley, Jezia

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 221, 536 231, 536 243, 536 2532, 536 253, 536 266, C12Q 168, C12P 1934, C07H 1900, C07H 1904, C07H 2104

Patent

active

061501077

ABSTRACT:
Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.

REFERENCES:
patent: 5723591 (1998-03-01), Livak et al.
Livak et al. "Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization" PCR Methods and Applications, pp. 357-362, 1995.

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